The accurate measurement of boundary flow field is very important for hydrodynamics research and aerospace applications. To this end, we propose a femtosecond laser-induced cyano chemiluminescence (FLICC) technique. The femtosecond laser was used to generate optical filaments in methane/nitrogen mixed flow field and induce CN (B-X) fluorescence with high intensity and long lifetime, thus realizing high spatial and temporal resolution flow field velocity measurement. Experimental results show that FLICC technique can be effectively used in complex flow and high spatial and temporal resolution measurement of boundary layer velocity is successfully achieved.