| 基于三维牵引力显微镜的力学测量研究 |
| Mechanical measurement study based on three-dimensional traction microscopy |
| 投稿时间：2024-12-04 修订日期：2024-12-26 |
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| 中文摘要: |
| 细胞通过细胞外基质感知其微环境中的硬度、弹性和拓扑结构等相关物理特性，并通过相关的力学敏感蛋白对其机械特性做出响应，进而改变其增殖、分化和迁移等行为。对细胞与细胞外基质之间机械力的高分辨测量对于研究细胞力学感知和力学信号转导起着至关重要的作用。本文通过采用结合了共聚焦显微镜和荧光微球标记物的牵引力显微镜（Traction Force Microscopy, TFM）技术，测量了肿瘤细胞对细胞外基质在三维空间内施加的牵引力，其具有高分率、非侵入性以及实时原位检测等优势。该技术为微纳尺度的力学测量提供了新思路。 |
| 英文摘要: |
| Cells sense the physical properties of their microenvironment such as stiffness, elasticity and topology through the extracellular matrix, and respond to the mechanical properties with related mechano-sensitive proteins, thereby changing their proliferation, differentiation and migration behaviors. High-resolution measurement of mechanical forces between cells and extracellular matrix plays a crucial role in studying cellular mechanosensing and mechanical signal transduction. In this paper, Traction Force Microscopy (TFM), which combines confocal microscopy and fluorescent microsphere markers, was used to measure the traction force of tumor cells on their extracellular matrix in three-dimensional space. TFM has the advantages of high resolution, non-invasive, and real-time in situ detection, which provides a new idea for micro- and nano-scale mechanical measurement. |
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| 中文关键词: 牵引力显微镜 力学测量 细胞力学 |
| 英文关键词:Traction microscopy Mechanical measurement Cell mechanics |
| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目） |
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