| 石墨烯涂覆锥形光纤的快速生物检测研究 |
| Research on rapid biosensing with graphene⁃coated conical optical fibers |
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| 中文摘要: |
| 为响应快速、高灵敏度检测结核病抗原及新型冠状病毒蛋白的需求，研发了一种检测结核病抗原（MPT64蛋白、 Ag85B蛋白）和严重急性呼吸综合症冠状病毒2 （Severe Acute Respiratory Syndrome Coronavirus 2， SARS?CoV?2）中核衣壳（Nucleocapsid， N）蛋白的高灵敏度光学微纤传感器。该光学微纤传感器通过具有极高表面积和优异光学特性，并能显著增强锥形光纤固定能力的氧化石墨烯（Graphene Oxide， GO）进行涂覆；然后采用能够在体外有效捕获目标蛋白，并实现实时检测的适配体单链DNA（Single?stranded DNA， ssDNA）对GO涂覆的锥形光纤进行修饰。采用制备好的传感器对目标物质进行检测。实验结果显示：该传感器能够在复杂样本中快速检测MPT64和Ag85B，检测时间仅为10 s，检测限（Limit of Detection， LOD）分别为4.23 × 10?2? M和3.11 × 10?1? M；另外，对SARS?CoV?2中N蛋白的LOD为6.25 × 10?1? M。该光学微纤传感器具备高灵敏度和快速检测的优势，有望在诸如结核病诊断和冠状病毒检测等医学领域发挥重要的作用。 |
| 英文摘要: |
| In response to the need for rapid and highly sensitive detection of tuberculosis antigens and novel coronavirus proteins, a highly?sensitivity optical microfiber sensor for detecting tuberculosis antigens (MPT64 protein, Ag85B protein) and the nucleocapsid (N) protein of severe acute respiratory syndrome coronavirus 2 (SARS?CoV?2) has been developed. The optical microfiber sensor is coated with graphene oxide (GO) which offers extremely high surface area and excellent optical properties, and can significantly enhance the conical optical fiber's immobilization capacity; the GO?coated conical optical fiber is further functionalized with single?stranded DNA (ssDNA) aptamers, enabling efficient capture of target proteins and facilitating real?time detection in vitro. The prepared sensor is employed to detect the target analytes. The experimental results reveal that the sensor can rapidly detect MPT64 and Ag85B in complex samples within 10 s, achieving detection limits of 4.23 × 10?2? M and 3.11 × 10?1? M, respectively. Additionally, the sensor exhibits a detection limit of 6.25 × 10?1? M for the N protein of SARS?CoV?2. The optical microfiber sensor possesses the advantages of high sensitivity and rapid detection, and is expected to play an important role in medical fields such as tuberculosis diagnosis and coronavirus detection. |
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| 中文关键词: 灵敏检测 锥形光纤传感器 冠状病毒 结核病 蛋白 |
| 英文关键词:sensitive detection tapered optical fiber sensor coronavirus tuberculosis protein |
| 基金项目: |
| DOI：10.11823/j.issn.1674-5795.2025.03.08 |
| 引用本文：刘忍, 王晶晶, 冯吉军.石墨烯涂覆锥形光纤的快速生物检测研究[J].计测技术,2025,45(3):85~99. |
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